FBXO22 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: FBXO22 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA854115ESR1HU
• Hersteller Artikelnummer: CSB-PA854115ESR1HU
• Alternativnummer: CSB-PA854115ESR1HU-100UL, CSB-PA854115ESR1HU-50UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Konjugation: Unconjugated
• Alternative Synonym: 0610033L19Rik antibody, 1600016C16Rik antibody, F box only protein 22 antibody, F box protein 22 antibody, F box protein FBX22p44 antibody, F-box only protein 22 antibody, F-box protein FBX22p44 antibody, FBX22 antibody, FBX22_HUMAN antibody, FBXO 22 antibody, FBXO22 antibody, FIST domain containing 1 antibody, FISTC1 antibody, FLJ13986 antibody, MGC124731 antibody, MGC31799 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q8NEZ5
• Puffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: FBXO22

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:1000-1:3000, IHC:1:100-1:300

Bilder

IHC image of CSB-PA854115ESR1HU diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA854115ESR1HU diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

Western Blot
Positive WB detected in:Mouse Brain tissue lysate(20µg)
All lanes: FBXO22 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 45kDa
Observed band size: 45 kDa
Exposure time: 10s

Western Blot
Positive WB detected in: Hela whole cell lysate(20µg), MCF7 whole cell lysate(20µg), HepG2 whole cell lysate(20µg), 293 whole cell lysate(20µg), JK whole cell lysate(20µg), K562 whole cell lysate(20µg), A549 whole cell lysate(20µg), A431 whole cell lysate(20µg), U251 whole cell lysate(20µg)
All lanes: FBXO22 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 10s