FBXO22 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA854115ESR1HU
Article Name: FBXO22 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA854115ESR1HU
Supplier Catalog Number: CSB-PA854115ESR1HU
Alternative Catalog Number: CSB-PA854115ESR1HU-100UL, CSB-PA854115ESR1HU-50UL
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, WB
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Alternative Names: 0610033L19Rik antibody, 1600016C16Rik antibody, F box only protein 22 antibody, F box protein 22 antibody, F box protein FBX22p44 antibody, F-box only protein 22 antibody, F-box protein FBX22p44 antibody, FBX22 antibody, FBX22_HUMAN antibody, FBXO 22 antibody, FBXO22 antibody, FIST domain containing 1 antibody, FISTC1 antibody, FLJ13986 antibody, MGC124731 antibody, MGC31799 antibody
Clonality: Polyclonal
UniProt: Q8NEZ5
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purity: Antigen Affinity Purified
Form: Liquid
Target: FBXO22
Application Dilute: Recommended dilution: WB:1:1000-1:3000, IHC:1:100-1:300
IHC image of CSB-PA854115ESR1HU diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody
IHC image of CSB-PA854115ESR1HU diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody
Western Blot
Positive WB detected in:Mouse Brain tissue lysate(20µg)
All lanes: FBXO22 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 45kDa
Observed band size: 45 kDa
Exposure time: 10s
Western Blot
Positive WB detected in: Hela whole cell lysate(20µg), MCF7 whole cell lysate(20µg), HepG2 whole cell lysate(20µg), 293 whole cell lysate(20µg), JK whole cell lysate(20µg), K562 whole cell lysate(20µg), A549 whole cell lysate(20µg), A431 whole cell lysate(20µg), U251 whole cell lysate(20µg)
All lanes: FBXO22 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 10s