MYO5A Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: MYO5A Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA857430LA01HU
• Hersteller Artikelnummer: CSB-PA857430LA01HU
• Alternativnummer: CSB-PA857430LA01HU-100UG, CSB-PA857430LA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IHC
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: non-muscle antibody, Dilute myosin heavy chain antibody, GS1 antibody, MYH12 antibody, MYO5 antibody, Myo5a antibody, MYO5A_HUMAN antibody, Myosin heavy chain 12 antibody, Myosin heavy polypeptide kinase antibody, Myosin V antibody, Myosin VA (heavy polypeptide 12 myoxin) antibody, Myosin VA (heavy polypeptide 12 myoxin) antibody, Myosin-12 antibody, Myoxin antibody, MYR12 antibody, Unconventional myosin-Va antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q9Y4I1
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: MYO5A

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: IHC:1:100-1:400

Bilder

IHC image of CSB-PA857430LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of A549 cells with CSB-PA857430LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate
All lanes: MYO5A antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 216, 213, 219 kDa
Observed band size: 216 kDa

Western Blot
Positive WB detected in: Mouse brain tissue
All lanes: MYO5A antibody at 7.3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 216, 213, 219 kDa
Observed band size: 216 kDa