MYO5A Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA857430LA01HU
- Images (4)
| Article Name: | MYO5A Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA857430LA01HU |
| Supplier Catalog Number: | CSB-PA857430LA01HU |
| Alternative Catalog Number: | CSB-PA857430LA01HU-100UG, CSB-PA857430LA01HU-50UG |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IHC |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | non-muscle antibody, Dilute myosin heavy chain antibody, GS1 antibody, MYH12 antibody, MYO5 antibody, Myo5a antibody, MYO5A_HUMAN antibody, Myosin heavy chain 12 antibody, Myosin heavy polypeptide kinase antibody, Myosin V antibody, Myosin VA (heavy polypeptide 12 myoxin) antibody, Myosin VA (heavy polypeptide 12 myoxin) antibody, Myosin-12 antibody, Myoxin antibody, MYR12 antibody, Unconventional myosin-Va antibody |
| Clonality: | Polyclonal |
| UniProt: | Q9Y4I1 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purity: | >95%, Protein G purified |
| Form: | Liquid |
| Target: | MYO5A |
| Application Dilute: | Recommended dilution: IHC:1:100-1:400 |
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IHC image of CSB-PA857430LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Immunofluorescence staining of A549 cells with CSB-PA857430LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Western Blot Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate All lanes: MYO5A antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 216, 213, 219 kDa Observed band size: 216 kDa |
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Western Blot Positive WB detected in: Mouse brain tissue All lanes: MYO5A antibody at 7.3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 216, 213, 219 kDa Observed band size: 216 kDa |
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