SMO Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: SMO Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA857876LA01HU
• Hersteller Artikelnummer: CSB-PA857876LA01HU
• Alternativnummer: CSB-PA857876LA01HU-100UG, CSB-PA857876LA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Konjugation: Unconjugated
• Alternative Synonym: Frizzled family member 11 antibody, FZD11 antibody, Gx antibody, Gx protein antibody, Protein Gx antibody, SMO antibody, SMO_HUMAN antibody, SMOH antibody, Smoothened frizzled family receptor antibody, Smoothened homolog (Drosophila) antibody, Smoothened homolog antibody, Smoothened homolog precursor (SMO) (Gx protein) antibody, Smoothened seven transmembrane spanning receptor antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q99835
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: SMO

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Bilder

Immunofluorescence staining of A549 cell with CSB-PA857876LA01HU at 1:130, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of SY5Y cell with CSB-PA857876LA01HU at 1:130, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of SY5Y cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, JK whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, Mouse Brain tissue lysate, Rat Brain tissue lysate
All lanes: SMO antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDa