SMO Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SMO Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA857876LA01HU
• Supplier Catalog Number: CSB-PA857876LA01HU
• Alternative Catalog Number: CSB-PA857876LA01HU-100UG, CSB-PA857876LA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: Frizzled family member 11 antibody, FZD11 antibody, Gx antibody, Gx protein antibody, Protein Gx antibody, SMO antibody, SMO_HUMAN antibody, SMOH antibody, Smoothened frizzled family receptor antibody, Smoothened homolog (Drosophila) antibody, Smoothened homolog antibody, Smoothened homolog precursor (SMO) (Gx protein) antibody, Smoothened seven transmembrane spanning receptor antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q99835
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: SMO

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Images

Immunofluorescence staining of A549 cell with CSB-PA857876LA01HU at 1:130, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of SY5Y cell with CSB-PA857876LA01HU at 1:130, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of SY5Y cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, JK whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, Mouse Brain tissue lysate, Rat Brain tissue lysate
All lanes: SMO antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDa