Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA875672ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA875672ESR1HU at dilution of 1:100
Immunofluorescent analysis of A549 cells using CSB-PA875672ESR1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IHC image of CSB-PA875672ESR1HU diluted at 1:364 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: 293T whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate All lanes: SAV1 antibody at 3.64µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa
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