SAV1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SAV1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA875672ESR1HU
• Supplier Catalog Number: CSB-PA875672ESR1HU
• Alternative Catalog Number: CSB-PA875672ESR1HU-100UL, CSB-PA875672ESR1HU-50UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: 1700040G09Rik antibody, 45 kDa WW domain protein antibody, hWW 45 antibody, hWW45 antibody, Protein salvador homolog 1 antibody, salvador family WW domain containing protein 1 antibody, salvador homolog 1 (Drosophila) antibody, Salvador homolog 1 antibody, Salvador, Drosophila, homolog of antibody, SAV 1 antibody, SAV antibody, SAV1 antibody, SAV1_HUMAN antibody, WW 45 antibody, WW domain containing antibody, WW domain-containing adaptor 45 antibody, WW domain-containing protein, 45-KD antibody, WW45 antibody, WW45 protein antibody, WWP 4 antibody, WWP4 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q9H4B6
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: SAV1

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:500, IF:1:50-1:200

Images

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA875672ESR1HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA875672ESR1HU at dilution of 1:100

Immunofluorescent analysis of A549 cells using CSB-PA875672ESR1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

IHC image of CSB-PA875672ESR1HU diluted at 1:364 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: 293T whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate
All lanes: SAV1 antibody at 3.64µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa