HAO2 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA882145LA01HU
- Bilder (6)
| Artikelname: | HAO2 Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA882145LA01HU |
| Hersteller Artikelnummer: | CSB-PA882145LA01HU |
| Alternativnummer: | CSB-PA882145LA01HU-100UG, CSB-PA882145LA01HU-50UG |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IHC, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Konjugation: | Unconjugated |
| Alternative Synonym: | (S)-2-hydroxy-acid oxidase antibody, (S)-2-hydroxy-acid oxidase, peroxisomal antibody, 2 hydroxy acid oxidase peroxisomal antibody, Cell growth-inhibiting gene 16 protein antibody, GIG16 antibody, Glycolate oxidase antibody, HAO 2 antibody, Hao2 antibody, HAOX2 antibody, HAOX2_HUMAN antibody, Hydroxyacid oxidase 2 (long chain) antibody, Hydroxyacid oxidase 2 antibody, Long chain alpha hydroxy acid oxidase antibody, Long chain alpha-hydroxy acid oxidase antibody, Long chain L 2 hydroxy acid oxidase antibody, Long-chain L-2-hydroxy acid oxidase antibody, peroxisomal antibody |
| Klonalität: | Polyclonal |
| UniProt: | Q9NYQ3 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Reinheit: | >95%, Protein G purified |
| Formulierung: | Liquid |
| Target-Kategorie: | HAO2 |
| Application Verdünnung: | Recommended dilution: WB:1:1000-1:3000, IHC:1:100-1:300 |
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IHC image of CSB-PA882145LA01HU diluted at 1:300 and staining in paraffin-embedded human Liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody |
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IHC image of CSB-PA882145LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody |
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IHC image of CSB-PA882145LA01HU diluted at 1:300 and staining in paraffin-embedded human Liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody |
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Western Blot Positive WB detected in: MCF7 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), A2780 whole cell lysate(30µg), PC-3 whole cell lysate(30µg),Mouse Kidney tissue lysate(30µg),Mouse Liver tissue lysate(30µg),Rat Kidney tissue lysate(30µg) All lanes: HAO2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 39, 41 kDa Observed band size: 39 kDa Exposure time: 60s |
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