HAO2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA882145LA01HU
Article Name: HAO2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA882145LA01HU
Supplier Catalog Number: CSB-PA882145LA01HU
Alternative Catalog Number: CSB-PA882145LA01HU-100UG, CSB-PA882145LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, WB
Species Reactivity: Human, Mouse, Rat
Conjugation: Unconjugated
Alternative Names: (S)-2-hydroxy-acid oxidase antibody, (S)-2-hydroxy-acid oxidase, peroxisomal antibody, 2 hydroxy acid oxidase peroxisomal antibody, Cell growth-inhibiting gene 16 protein antibody, GIG16 antibody, Glycolate oxidase antibody, HAO 2 antibody, Hao2 antibody, HAOX2 antibody, HAOX2_HUMAN antibody, Hydroxyacid oxidase 2 (long chain) antibody, Hydroxyacid oxidase 2 antibody, Long chain alpha hydroxy acid oxidase antibody, Long chain alpha-hydroxy acid oxidase antibody, Long chain L 2 hydroxy acid oxidase antibody, Long-chain L-2-hydroxy acid oxidase antibody, peroxisomal antibody
Clonality: Polyclonal
UniProt: Q9NYQ3
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: HAO2
Application Dilute: Recommended dilution: WB:1:1000-1:3000, IHC:1:100-1:300
IHC image of CSB-PA882145LA01HU diluted at 1:300 and staining in paraffin-embedded human Liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody
IHC image of CSB-PA882145LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody
IHC image of CSB-PA882145LA01HU diluted at 1:300 and staining in paraffin-embedded human Liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody
Western Blot
Positive WB detected in: MCF7 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), A2780 whole cell lysate(30µg), PC-3 whole cell lysate(30µg),Mouse Kidney tissue lysate(30µg),Mouse Liver tissue lysate(30µg),Rat Kidney tissue lysate(30µg)
All lanes: HAO2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 39, 41 kDa
Observed band size: 39 kDa
Exposure time: 60s