AGO2 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: AGO2 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA891731LA01HU
• Hersteller Artikelnummer: CSB-PA891731LA01HU
• Alternativnummer: CSB-PA891731LA01HU-100UG, CSB-PA891731LA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Konjugation: Unconjugated
• Alternative Synonym: Ago 2 antibody, AGO2_HUMAN antibody, Argonaute 2 antibody, argonaute 2, RISC catalytic component antibody, Argonaute RISC catalytic component 2 antibody, Argonaute2 antibody, CTA-204B4.6 antibody, dAgo2 antibody, eIF 2C 2 antibody, eIF-2C 2 antibody, eIF2C 2 antibody, Eif2c2 antibody, Eukaryotic translation initiation factor 2C 2 antibody, Eukaryotic translation initiation factor 2C subunit 2 antibody, hAgo2 antibody, MGC3183 antibody, PAZ Piwi domain protein antibody, PPD antibody, Protein argonaute-2 antibody, Protein slicer antibody, Q10 antibody, Slicer protein antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q9UKV8
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: AGO2

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:1000-1:5000, IHC:1:100-1:1500

Bilder

IHC image of CSB-PA891731LA01HU diluted at 1:800 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA891731LA01HU diluted at 1:800 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA891731LA01HU diluted at 1:800 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

Western Blot
Positive WB detected in: JK whole cell lysate(20µg), HepG2 whole cell lysate(20µg), K562 whole cell lysate(20µg), PC-3 whole cell lysate(20µg)
All lanes: AGO2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 98 kDa
Observed band size: 98 kDa
Exposure time:1min