LDLR Recombinant Monoclonal Antibody, Clone: [2B10], Unconjugated, Rabbit

Produktübersicht

• Artikelname: LDLR Recombinant Monoclonal Antibody, Clone: [2B10], Unconjugated, Rabbit
• Artikelnummer: CSB-RA575353A0HU
• Hersteller Artikelnummer: CSB-RA575353A0HU
• Alternativnummer: CSB-RA575353A0HU-100UL, CSB-RA575353A0HU-50UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: Low-density lipoprotein receptor (LDL receptor) , LDLR

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [2B10]
• UniProt: P01130
• Puffer: Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Reinheit: Affinity-chromatography
• Formulierung: Liquid
• Target-Kategorie: LDLR
• Antibody Type: Recombinant Antibody

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:1000-1:3000, IF:1:20-1:100

Bilder

Immunofluorescence staining of Hela cell with CSB-RA575353A0HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .

Immunofluorescence staining of HepG2 cell with CSB-RA575353A0HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .

Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .

Western Blot
Positive WB detected in: Hela whole cell lysate(30µg) , JK whole cell lysate(30µg) , SY5Y whole cell lysate(30µg)
All lanes: LDLR antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 96 kDa
Observed band size: 100,140 kDa
Exposure time: 60s