LDLR Recombinant Monoclonal Antibody, Clone: [2B10], Unconjugated, Rabbit

Catalog Number: CSB-RA575353A0HU
Article Name: LDLR Recombinant Monoclonal Antibody, Clone: [2B10], Unconjugated, Rabbit
Biozol Catalog Number: CSB-RA575353A0HU
Supplier Catalog Number: CSB-RA575353A0HU
Alternative Catalog Number: CSB-RA575353A0HU-100UL, CSB-RA575353A0HU-50UL
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: Low-density lipoprotein receptor (LDL receptor) , LDLR
Clonality: Monoclonal
Clone Designation: [2B10]
UniProt: P01130
Buffer: Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Form: Liquid
Target: LDLR
Antibody Type: Recombinant Antibody
Application Dilute: Recommended dilution: WB:1:1000-1:3000, IF:1:20-1:100
Immunofluorescence staining of Hela cell with CSB-RA575353A0HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .
Immunofluorescence staining of HepG2 cell with CSB-RA575353A0HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .
Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .
Western Blot
Positive WB detected in: Hela whole cell lysate(30µg) , JK whole cell lysate(30µg) , SY5Y whole cell lysate(30µg)
All lanes: LDLR antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 96 kDa
Observed band size: 100,140 kDa
Exposure time: 60s