Goat F(ab)2 anti-Human IgA (alpha chain) Antibody alkaline phosphatase Conjugation, Goat F(ab)2 anti-Human IgA alpha alk phos Conjugated Antibody
Format:
F(ab')2
Spezifität:
IgA
Minimale Kreuzreaktivität (MinX):
no cross-adsorbtion
F(ab)2 Anti-Human IgA Alkaline Phosphatase Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can
Klonalität:
Polyclonal
Konzentration:
0.7 mg/mL
Isotyp:
Ig
Puffer:
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Reinheit:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Human IgA and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Formulierung:
Lyophilized
Formel:
100 mM Tric-HCl,500 mM NaCl,pH8,0,lyophilisate,0.05% NaN3
Target-Kategorie:
Human
Antibody Type:
Secondary Antibody
Application Verdünnung:
ELISA Dilution: 1:2,000-1:10,000, Immunohistochemistry Dilution: 1:200-1:1,000, Western Blot Dilution: 1:500-1:2,500
Anwendungsbeschreibung:
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Human IgA in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
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