Goat F(ab)2 anti-Human IgA-Alk. Phos., MinX none, ALP, Polyclonal , AP

Catalog Number: DNA-SEC-183745
Article Name: Goat F(ab)2 anti-Human IgA-Alk. Phos., MinX none, ALP, Polyclonal , AP
Biozol Catalog Number: DNA-SEC-183745
Supplier Catalog Number: SEC-183745
Alternative Catalog Number: DNA-SEC-183745
Manufacturer: dianova
Host: Goat
Category: Antikörper
Application: ELISA,IHC,WB
Species Reactivity: Human
Immunogen: Human IgA alpha heavy chain
Conjugation: Alk. Phos.
Alternative Names: Goat F(ab)2 anti-Human IgA (alpha chain) Antibody alkaline phosphatase Conjugation, Goat F(ab)2 anti-Human IgA alpha alk phos Conjugated Antibody
Format: F(ab')2
Target Specificity: IgA
Cross-Adsorption (MinX): no cross-adsorbtion
F(ab)2 Anti-Human IgA Alkaline Phosphatase Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can
Clonality: Polyclonal
Concentration: 0.7 mg/mL
Isotype: Ig
Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Human IgA and Human Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).  Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Form: Lyophilized
Formula: 100 mM Tric-HCl,500 mM NaCl,pH8,0,lyophilisate,0.05% NaN3
Target: Human
Antibody Type: Secondary Antibody
Application Dilute: ELISA Dilution: 1:2,000-1:10,000, Immunohistochemistry Dilution: 1:200-1:1,000, Western Blot Dilution: 1:500-1:2,500
Application Notes: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Human IgA in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.