EasyStep Rat ADP(Adiponectin) ELISA Kit

Artikelnummer: EBT-ELK030ES
Artikelname: EasyStep Rat ADP(Adiponectin) ELISA Kit
Artikelnummer: EBT-ELK030ES
Hersteller Artikelnummer: ELK030ES
Alternativnummer: EBT-ELK030ES-96, EBT-ELK030ES-48, EBT-ELK030ES-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Rat
Alternative Synonym: GBP28, ApM1, AdipoQ, Acrp30, ACDC, APM1, C1Q And Collagen Domain Containing, Adipocyte Complement-Related Protein Of 30 KDa, Adipose Most Abundant Gene Transcript 1
Konzentration: 500 ng/mL
Detektionsbereich: 7.82-500 ng/mL
Sensitivitaet: 4.75 ng/mL
Proben: Serum, plasma
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 1.5h. Research Area: Metabolic pathway,Infection immunity,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADP, and the Rat ADP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat ADP. After TMB substrate solution is added, only those wells that contain Rat ADP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat ADP in the samples is then determined by comparing the OD of the samples to the standard curve