EasyStep Rat ADP(Adiponectin) ELISA Kit

Catalog Number: EBT-ELK030ES
Article Name: EasyStep Rat ADP(Adiponectin) ELISA Kit
Biozol Catalog Number: EBT-ELK030ES
Supplier Catalog Number: ELK030ES
Alternative Catalog Number: EBT-ELK030ES-96, EBT-ELK030ES-48, EBT-ELK030ES-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Rat
Alternative Names: GBP28, ApM1, AdipoQ, Acrp30, ACDC, APM1, C1Q And Collagen Domain Containing, Adipocyte Complement-Related Protein Of 30 KDa, Adipose Most Abundant Gene Transcript 1
Concentration: 500 ng/mL
Range: 7.82-500 ng/mL
Sensitivity: 4.75 ng/mL
Samples: Serum, plasma
Application Notes: Assay Type: Sandwich. Assay length: 1.5h. Research Area: Metabolic pathway,Infection immunity,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADP, and the Rat ADP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat ADP. After TMB substrate solution is added, only those wells that contain Rat ADP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat ADP in the samples is then determined by comparing the OD of the samples to the standard curve