Human Anti-tTG IgG (Anti-Tissue Transglutaminase IgG) ELISA Kit

Produktübersicht

• Artikelname: Human Anti-tTG IgG (Anti-Tissue Transglutaminase IgG) ELISA Kit
• Artikelnummer: EBT-ELK0751
• Hersteller Artikelnummer: ELK0751
• Alternativnummer: EBT-ELK0751-96, EBT-ELK0751-48, EBT-ELK0751-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Human
• Alternative Synonym: Anti-Tissue Transglutaminase IgG

Produkteigenschaften

• Konzentration: 100 ng/mL
• Detektionsbereich: 1.56-100 ng/mL
• Sensitivitaet: 0.6 ng/mL
• Proben: Serum, plasma, tissue homogenates and other biological fluids.

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,Endocrinology,Neuro science,Autoimmunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human Anti-tTG IgG in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK0751