Human Anti-tTG IgG (Anti-Tissue Transglutaminase IgG) ELISA Kit
Biozol Catalog Number:
EBT-ELK0751
Supplier Catalog Number:
ELK0751
Alternative Catalog Number:
EBT-ELK0751-96, EBT-ELK0751-48, EBT-ELK0751-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Human
Alternative Names:
Anti-Tissue Transglutaminase IgG
Concentration:
100 ng/mL
Range:
1.56-100 ng/mL
Sensitivity:
0.6 ng/mL
Samples:
Serum, plasma, tissue homogenates and other biological fluids.
Application Notes:
Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,Endocrinology,Neuro science,Autoimmunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human Anti-tTG IgG in the samples is then determined by comparing the OD of the samples to the standard curve
ELK0751
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