Cattle anti-HAV IgG(anti-Hepatitis A antibody IgG) ELISA Kit

Produktübersicht

• Artikelname: Cattle anti-HAV IgG(anti-Hepatitis A antibody IgG) ELISA Kit
• Artikelnummer: EBT-ELK0762
• Hersteller Artikelnummer: ELK0762
• Alternativnummer: EBT-ELK0762-96,EBT-ELK0762-48,EBT-ELK0762-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Bovine
• Alternative Synonym: Hepatitis A virus IgG

Produkteigenschaften

• Konzentration: 10 ng/mL
• Detektionsbereich: 0.16-10 ng/mL
• Sensitivitaet: 0.069 ng/mL
• Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle Anti-HAV IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle Anti-HAV IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle Anti-HAV IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Cattle Anti-HAV IgG in the samples is then determined by comparing the OD of the samples to the standard curve