Cattle anti-HAV IgG(anti-Hepatitis A antibody IgG) ELISA Kit
Biozol Catalog Number:
EBT-ELK0762
Supplier Catalog Number:
ELK0762
Alternative Catalog Number:
EBT-ELK0762-96,EBT-ELK0762-48,EBT-ELK0762-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Bovine
Alternative Names:
Hepatitis A virus IgG
Concentration:
10 ng/mL
Range:
0.16-10 ng/mL
Sensitivity:
0.069 ng/mL
Samples:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Assay Type: Sandwich. Assay length: 3.5h. Research Area: Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle Anti-HAV IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle Anti-HAV IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle Anti-HAV IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Cattle Anti-HAV IgG in the samples is then determined by comparing the OD of the samples to the standard curve
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