Rat NFkappaB-p65(Nuclear Factor Kappa B p65) ELISA Kit

Produktübersicht

• Artikelname: Rat NFkappaB-p65(Nuclear Factor Kappa B p65) ELISA Kit
• Artikelnummer: EBT-ELK1693
• Hersteller Artikelnummer: ELK1693
• Alternativnummer: EBT-ELK1693-96,EBT-ELK1693-48,EBT-ELK1693-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Rat
• Alternative Synonym: NFKB3, p65, REL-A, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3, Transcription Factor P65, Nuclear factor NF-kappa-B p65 subunit, RELA, V-Rel Reticuloendotheliosis Viral Oncogene Homolog A

Produkteigenschaften

• Konzentration: 10 ng/mL
• Detektionsbereich: 0.16-10 ng/mL
• Sensitivitaet: 0.055 ng/mL
• Proben: Tissue homogenates, cell lysates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Apoptosis,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NFkappaB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NFkappaB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NFkappaB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat NFkappaB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve