Rat NFkappaB-p65(Nuclear Factor Kappa B p65) ELISA Kit
Biozol Catalog Number:
EBT-ELK1693
Supplier Catalog Number:
ELK1693
Alternative Catalog Number:
EBT-ELK1693-96,EBT-ELK1693-48,EBT-ELK1693-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Rat
Alternative Names:
NFKB3, p65, REL-A, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3, Transcription Factor P65, Nuclear factor NF-kappa-B p65 subunit, RELA, V-Rel Reticuloendotheliosis Viral Oncogene Homolog A
Concentration:
10 ng/mL
Range:
0.16-10 ng/mL
Sensitivity:
0.055 ng/mL
Samples:
Tissue homogenates, cell lysates and other biological fluids
Application Notes:
Assay Type: Sandwich. Assay length: 3.5h. Research Area: Apoptosis,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NFkappaB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NFkappaB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NFkappaB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat NFkappaB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve
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