Human CCK8(Cholecystokinin 8, Octapeptide) ELISA Kit

Artikelnummer: EBT-ELK1745
Artikelname: Human CCK8(Cholecystokinin 8, Octapeptide) ELISA Kit
Artikelnummer: EBT-ELK1745
Hersteller Artikelnummer: ELK1745
Alternativnummer: EBT-ELK1745-96, EBT-ELK1745-48, EBT-ELK1745-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: Sincalide
Konzentration: 1000 pg/mL
Detektionsbereich: 15.63-1000 pg/mL
Sensitivitaet: 4.46 pg/mL
Proben: Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Assay Type: Competitive Inhibition. Assay length: 2h. Research Area: Endocrinology,Gastroenterology,Hormone metabolism,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CCK8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCK8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human CCK8 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK1745