Human CCK8(Cholecystokinin 8, Octapeptide) ELISA Kit
Biozol Catalog Number:
EBT-ELK1745
Supplier Catalog Number:
ELK1745
Alternative Catalog Number:
EBT-ELK1745-96, EBT-ELK1745-48, EBT-ELK1745-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Human
Alternative Names:
Sincalide
Concentration:
1000 pg/mL
Range:
15.63-1000 pg/mL
Sensitivity:
4.46 pg/mL
Samples:
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Assay Type: Competitive Inhibition. Assay length: 2h. Research Area: Endocrinology,Gastroenterology,Hormone metabolism,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CCK8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCK8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human CCK8 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK1745
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