Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit

Produktübersicht

• Artikelname: Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit
• Artikelnummer: EBT-ELK4734
• Hersteller Artikelnummer: ELK4734
• Alternativnummer: EBT-ELK4734-96, EBT-ELK4734-48, EBT-ELK4734-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Human
• Alternative Synonym: NFH, 200 kDa neurofilament protein, Neurofilament triplet H protein, NEFH, Neurofilament, Heavy Polypeptide

Produkteigenschaften

• Konzentration: 1000 pg/mL
• Detektionsbereich: 15.63-1000 pg/mL
• Sensitivitaet: 5.7 pg/mL
• Proben: serum, plasma, tissue homogenates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Neuro science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human pNF-H. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human pNF-H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pNF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human pNF-H in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK4734