Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit
Biozol Catalog Number:
EBT-ELK4734
Supplier Catalog Number:
ELK4734
Alternative Catalog Number:
EBT-ELK4734-96, EBT-ELK4734-48, EBT-ELK4734-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Human
Alternative Names:
NFH, 200 kDa neurofilament protein, Neurofilament triplet H protein, NEFH, Neurofilament, Heavy Polypeptide
Concentration:
1000 pg/mL
Range:
15.63-1000 pg/mL
Sensitivity:
5.7 pg/mL
Samples:
serum, plasma, tissue homogenates and other biological fluids
Application Notes:
Assay Type: Sandwich. Assay length: 3.5h. Research Area: Neuro science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human pNF-H. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human pNF-H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pNF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human pNF-H in the samples is then determined by comparing the OD of the samples to the standard curve
ELK4734
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