Human 15-HETE(15-Hydroxyeicosatetraenoic Acid) ELISA Kit

Produktübersicht

• Artikelname: Human 15-HETE(15-Hydroxyeicosatetraenoic Acid) ELISA Kit
• Artikelnummer: EBT-ELK8351
• Hersteller Artikelnummer: ELK8351
• Alternativnummer: EBT-ELK8351-96,EBT-ELK8351-48,EBT-ELK8351-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Human
• Alternative Synonym: 15-HETE, 15-Hydroxyeicosatetraenoic Acid)

Produkteigenschaften

• Konzentration: 8 ng/mL
• Detektionsbereich: 0.13-8 ng/mL
• Sensitivitaet: 0.1 ng/mL
• Proben: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Competitive Inhibition. Assay length: 2h. Research Area: Signal transduction,Metabolic pathway,Infection immunity,Hormone metabolism,Dermatology,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 15-HETE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 15-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human 15-HETE in the samples is then determined by comparing the OD of the samples to the standard curve