Human 15-HETE(15-Hydroxyeicosatetraenoic Acid) ELISA Kit
Biozol Catalog Number:
EBT-ELK8351
Supplier Catalog Number:
ELK8351
Alternative Catalog Number:
EBT-ELK8351-96,EBT-ELK8351-48,EBT-ELK8351-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Human
Alternative Names:
15-HETE, 15-Hydroxyeicosatetraenoic Acid)
Concentration:
8 ng/mL
Range:
0.13-8 ng/mL
Sensitivity:
0.1 ng/mL
Samples:
serum, plasma, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Assay Type: Competitive Inhibition. Assay length: 2h. Research Area: Signal transduction,Metabolic pathway,Infection immunity,Hormone metabolism,Dermatology,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 15-HETE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 15-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human 15-HETE in the samples is then determined by comparing the OD of the samples to the standard curve
* VAT and and shipping costs not included. Errors and price changes excepted
