Mouse SP-A1(Pulmonarysurfactant-associated protein A1) ELISA Kit

Produktübersicht

• Artikelname: Mouse SP-A1(Pulmonarysurfactant-associated protein A1) ELISA Kit
• Artikelnummer: EBT-ELK9615
• Hersteller Artikelnummer: ELK9615
• Alternativnummer: EBT-ELK9615-96, EBT-ELK9615-48, EBT-ELK9615-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Mouse
• Alternative Synonym: MGC133365, PSAP, PSPA, SFTP1, SFTPA1

Produkteigenschaften

• Konzentration: 20 ng/mL
• Detektionsbereich: 0.31-20 ng/mL
• Sensitivitaet: 0.19 ng/mL
• Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal Transduction. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SP-A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SP-A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SP-A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse SP-A1 in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK9615