Mouse SP-A1(Pulmonarysurfactant-associated protein A1) ELISA Kit
Biozol Catalog Number:
EBT-ELK9615
Supplier Catalog Number:
ELK9615
Alternative Catalog Number:
EBT-ELK9615-96, EBT-ELK9615-48, EBT-ELK9615-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Mouse
Alternative Names:
MGC133365, PSAP, PSPA, SFTP1, SFTPA1
Concentration:
20 ng/mL
Range:
0.31-20 ng/mL
Sensitivity:
0.19 ng/mL
Samples:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal Transduction. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SP-A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SP-A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SP-A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse SP-A1 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK9615
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