Dog GFAP(Glial Fibrillary Acidic Protein) ELISA Kit

Produktübersicht

• Artikelname: Dog GFAP(Glial Fibrillary Acidic Protein) ELISA Kit
• Artikelnummer: EBT-ELK9719
• Hersteller Artikelnummer: ELK9719
• Alternativnummer: EBT-ELK9719-96, EBT-ELK9719-48, EBT-ELK9719-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Canine
• Alternative Synonym: Intermediate Filament Protein

Produkteigenschaften

• Konzentration: 10 ng/mL
• Detektionsbereich: 0.16-10 ng/mL
• Sensitivitaet: 0.055 ng/mL
• Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Infection immunity,Neuro science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog GFAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog GFAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog GFAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Dog GFAP in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK9719