Dog GFAP(Glial Fibrillary Acidic Protein) ELISA Kit
Biozol Catalog Number:
EBT-ELK9719
Supplier Catalog Number:
ELK9719
Alternative Catalog Number:
EBT-ELK9719-96, EBT-ELK9719-48, EBT-ELK9719-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Canine
Alternative Names:
Intermediate Filament Protein
Concentration:
10 ng/mL
Range:
0.16-10 ng/mL
Sensitivity:
0.055 ng/mL
Samples:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Assay Type: Sandwich. Assay length: 3.5h. Research Area: Infection immunity,Neuro science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog GFAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog GFAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog GFAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Dog GFAP in the samples is then determined by comparing the OD of the samples to the standard curve
ELK9719
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