DDDDK-Tag(binds to flag sequnence) rabbit pAb, Rabbit, Polyclonal

Produktübersicht

• Artikelname: DDDDK-Tag(binds to flag sequnence) rabbit pAb, Rabbit, Polyclonal
• Artikelnummer: EBT-ES1078
• Hersteller Artikelnummer: ES1078
• Alternativnummer: EBT-ES1078-50, EBT-ES1078-100
• Hersteller: ELK Biotechnology
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IP, WB
• Spezies Reaktivität: All
• Immunogen: DDDDK synthetic peptide conjugated to KLH.
• Alternative Synonym: DDDDK epitope tag, DDDDK epitope tag, DYKDDDDK epitope tag

Produktbeschreibung

The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml

Anwendungsbeschreibung

• Application Verdünnung: Western Blot: 1/1000 - 1/3000. ELISA: 1:5000-20000. Not yet tested in other applications.

Bilder

Western Blot analysis using FLAG-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing FLAG tag (1) and untransfected (2). Secondary antibody(catalog:RS0002) was diluted at 1:20000