DDDDK-Tag(binds to flag sequnence) rabbit pAb, Rabbit, Polyclonal
Biozol Catalog Number:
EBT-ES1078
Supplier Catalog Number:
ES1078
Alternative Catalog Number:
EBT-ES1078-50, EBT-ES1078-100
Manufacturer:
ELK Biotechnology
Host:
Rabbit
Category:
Antikörper
Application:
ELISA, IF, IP, WB
Species Reactivity:
All
Immunogen:
DDDDK synthetic peptide conjugated to KLH.
Alternative Names:
DDDDK epitope tag, DDDDK epitope tag, DYKDDDDK epitope tag
The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
Clonality:
Polyclonal
Concentration:
1 mg/ml
Application Dilute:
Western Blot: 1/1000 - 1/3000. ELISA: 1:5000-20000. Not yet tested in other applications.
Western Blot analysis using FLAG-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing FLAG tag (1) and untransfected (2). Secondary antibody(catalog:RS0002) was diluted at 1:20000
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