The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Western Blot analysis of various cells using Phospho-PKD2 (S876) Polyclonal Antibody diluted at 1:1000
Western Blot analysis of HELA cells using Phospho-PKD2 (S876) Polyclonal Antibody diluted at 1:1000
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKD2 (Phospho-Ser876) Antibody
Western blot analysis of lysates from NIH/3T3 cells treated with PMA 250ng/ml 15, using PKD2 (Phospho-Ser876) Antibody. The lane on the right is blocked with the phospho peptide.
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