Crk II (phospho Tyr221) rabbit pAb, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Crk II (phospho Tyr221) rabbit pAb, Rabbit, Polyclonal
• Artikelnummer: EBT-ES1448
• Hersteller Artikelnummer: ES1448
• Alternativnummer: EBT-ES1448-50, EBT-ES1448-100
• Hersteller: ELK Biotechnology
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, WB
• Spezies Reaktivität: Human, Monkey, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human CrkII around the phosphorylation site of Tyr221. AA range:187-236
• Alternative Synonym: CRK, Adapter molecule crk, Proto-oncogene c-Crk, p38

Produktbeschreibung

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008],

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml
• Molekulargewicht: 40kD
• NCBI: 1398
• UniProt: P46108

Anwendungsbeschreibung

• Application Verdünnung: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Bilder

Western Blot analysis of various cells using Phospho-Crk II (Y221) Polyclonal Antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CrkII (Phospho-Tyr221) Antibody

Immunofluorescence analysis of HUVEC cells, using CrkII (Phospho-Tyr221) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells, using CrkII (Phospho-Tyr221) Antibody. The lane on the right is blocked with the phospho peptide.