Crk II (phospho Tyr221) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: Crk II (phospho Tyr221) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1448
• Supplier Catalog Number: ES1448
• Alternative Catalog Number: EBT-ES1448-50, EBT-ES1448-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, WB
• Species Reactivity: Human, Monkey, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human CrkII around the phosphorylation site of Tyr221. AA range:187-236
• Alternative Names: CRK, Adapter molecule crk, Proto-oncogene c-Crk, p38

Product Description

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 40kD
• NCBI: 1398
• UniProt: P46108

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Phospho-Crk II (Y221) Polyclonal Antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CrkII (Phospho-Tyr221) Antibody

Immunofluorescence analysis of HUVEC cells, using CrkII (Phospho-Tyr221) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells, using CrkII (Phospho-Tyr221) Antibody. The lane on the right is blocked with the phospho peptide.