The antiserum was produced against synthesized peptide derived from human Synaptotagmin around the phosphorylation site of Ser309. AA range:276-325
Alternative Synonym:
SYT1, SVP65, SYT, Synaptotagmin-1, Synaptotagmin I, SytI, p65, SYT2, Synaptotagmin-2, Synaptotagmin II, SytII
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Western Blot analysis of various cells using Phospho-Synaptotagmin 1/2 (S309/306) Polyclonal Antibody
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Synaptotagmin (Phospho-Ser309) Antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Synaptotagmin (Phospho-Ser309) Antibody. The picture on the right is blocked with the phospho peptide.
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