Synaptotagmin 1/2 (phospho Ser309/306) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: Synaptotagmin 1/2 (phospho Ser309/306) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1460
• Supplier Catalog Number: ES1460
• Alternative Catalog Number: EBT-ES1460-50, EBT-ES1460-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human Synaptotagmin around the phosphorylation site of Ser309. AA range:276-325
• Alternative Names: SYT1, SVP65, SYT, Synaptotagmin-1, Synaptotagmin I, SytI, p65, SYT2, Synaptotagmin-2, Synaptotagmin II, SytII

Product Description

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 47kD
• NCBI: 6857
• UniProt: P21579

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Phospho-Synaptotagmin 1/2 (S309/306) Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Synaptotagmin (Phospho-Ser309) Antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Synaptotagmin (Phospho-Ser309) Antibody. The picture on the right is blocked with the phospho peptide.