Apaf-1-ALT rabbit pAb, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Apaf-1-ALT rabbit pAb, Rabbit, Polyclonal
• Artikelnummer: EBT-ES1672
• Hersteller Artikelnummer: ES1672
• Alternativnummer: EBT-ES1672-50, EBT-ES1672-100
• Hersteller: ELK Biotechnology
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human APAF-1-ALT. AA range:289-338
• Alternative Synonym: APAF1-interacting protein, APIP, APIP2, CGI-29 protein, MMRP19, dJ179L10.2, likely ortholog of mouse monocyte macrophage 19

Produktbeschreibung

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml
• Molekulargewicht: 27kD
• NCBI: 317
• UniProt: O14727

Anwendungsbeschreibung

• Application Verdünnung: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Bilder

Western Blot analysis of various cells using Apaf-1-ALT Polyclonal Antibody diluted at 1:500

Western Blot analysis of COLO205 cells using Apaf-1-ALT Polyclonal Antibody diluted at 1:500

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using APAF-1-ALT Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO205 cells, using APAF-1-ALT Antibody. The lane on the right is blocked with the synthesized peptide.