Apaf-1-ALT rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: Apaf-1-ALT rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1672
• Supplier Catalog Number: ES1672
• Alternative Catalog Number: EBT-ES1672-50, EBT-ES1672-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human APAF-1-ALT. AA range:289-338
• Alternative Names: APAF1-interacting protein, APIP, APIP2, CGI-29 protein, MMRP19, dJ179L10.2, likely ortholog of mouse monocyte macrophage 19

Product Description

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 27kD
• NCBI: 317
• UniProt: O14727

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Apaf-1-ALT Polyclonal Antibody diluted at 1:500

Western Blot analysis of COLO205 cells using Apaf-1-ALT Polyclonal Antibody diluted at 1:500

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using APAF-1-ALT Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO205 cells, using APAF-1-ALT Antibody. The lane on the right is blocked with the synthesized peptide.