HIF-1 alpha (3M10) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE12017
Produktübersicht
Artikelname:
HIF-1 alpha (3M10) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE12017
Hersteller Artikelnummer:
AMRe12017
Alternativnummer:
EKL-AMRE12017-50UL, EKL-AMRE12017-100UL, EKL-AMRE12017-200UL
Hersteller:
EnkiLife
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
FC, ICC, IHC, IP, WB
Spezies Reaktivität:
Human
Konjugation:
Unconjugated
Alternative Synonym:
HIF1, MOP1, PASD8, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF1A
Produktbeschreibung
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed:11292861, PubMed: 11566883, PubMed: 15465032, PubMed: 16973622, PubMed: 17610843, PubMed: 18658046, PubMed: 20624928, PubMed: 22009797, PubMed: 9887100, PubMed: 30125331). Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed: 11292861, PubMed: 11566883, PubMed: 15465032, PubMed: 16973622, PubMed: 17610843, PubMed: 20624928, PubMed: 22009797, PubMed: 9887100, PubMed: 30125331). Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed: 22009797). Heterodimerizes with ARNT, heterodimer binds to core DNA sequence 5-TACGTG-3 within the hypoxia response element (HRE) of target gene promoters (By similarity). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300 (PubMed: 9887100, PubMed: 16543236). Activity is enhanced by interaction with NCOA1 and/or NCOA2 (PubMed: 10594042). Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP (PubMed: 10202154, PubMed: 10594042). Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (PubMed: 19528298).
Produkteigenschaften
Klonalität:
Monoclonal
Molekulargewicht:
93kDa
NCBI:
3091
UniProt:
Q16665
Puffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Reinheit:
Affinity purification
Formulierung:
Liquid
Target-Kategorie:
HIF1A
Anwendungsbeschreibung
Application Verdünnung:
WB 1:500-1:2000,IHC 1:50-1:200,ICC/IF 1:200-1:500,FC 1:500-1:2000,IP 1:50-1:200
