HIF-1 alpha (3M10) Rabbit Monoclonal Antibody, Unconjugated
Catalog Number:
EKL-AMRE12017
Product Overview
Article Name:
HIF-1 alpha (3M10) Rabbit Monoclonal Antibody, Unconjugated
Biozol Catalog Number:
EKL-AMRE12017
Supplier Catalog Number:
AMRe12017
Alternative Catalog Number:
EKL-AMRE12017-50UL, EKL-AMRE12017-100UL, EKL-AMRE12017-200UL
Manufacturer:
EnkiLife
Host:
Rabbit
Category:
Antikörper
Application:
FC, ICC, IHC, IP, WB
Species Reactivity:
Human
Conjugation:
Unconjugated
Alternative Names:
HIF1, MOP1, PASD8, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF1A
Product Description
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed:11292861, PubMed: 11566883, PubMed: 15465032, PubMed: 16973622, PubMed: 17610843, PubMed: 18658046, PubMed: 20624928, PubMed: 22009797, PubMed: 9887100, PubMed: 30125331). Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed: 11292861, PubMed: 11566883, PubMed: 15465032, PubMed: 16973622, PubMed: 17610843, PubMed: 20624928, PubMed: 22009797, PubMed: 9887100, PubMed: 30125331). Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed: 22009797). Heterodimerizes with ARNT, heterodimer binds to core DNA sequence 5-TACGTG-3 within the hypoxia response element (HRE) of target gene promoters (By similarity). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300 (PubMed: 9887100, PubMed: 16543236). Activity is enhanced by interaction with NCOA1 and/or NCOA2 (PubMed: 10594042). Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP (PubMed: 10202154, PubMed: 10594042). Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (PubMed: 19528298).
Product Properties
Clonality:
Monoclonal
Molecular Weight:
93kDa
NCBI:
3091
UniProt:
Q16665
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Purity:
Affinity purification
Form:
Liquid
Target:
HIF1A
Application Notes
Application Dilute:
WB 1:500-1:2000,IHC 1:50-1:200,ICC/IF 1:200-1:500,FC 1:500-1:2000,IP 1:50-1:200
