TNF alpha antibody

Artikelnummer: GTX-GTX110520
Artikelname: TNF alpha antibody
Artikelnummer: GTX-GTX110520
Hersteller Artikelnummer: GTX110520
Hersteller: Genetex
Wirt: Rabbit
Applikation: WB, IF, ICC, IHC-P
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Full length human TNF alpha Recombinant protein.
Konjugation: IgG
Alternative Synonym: Tumor Necrosis Factor,Dif,Tnf-Alpha,Tnfa,Tnfsf2,Tnlg1F,Tnf
Klonalität: Polyclonal
Konzentration: 0.04 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 26
Isotyp: IgG
NCBI: 7124
UniProt: P01375
Puffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Formulierung: Liquid
Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4-8ºC. For long-term storage, aliquot and store at -20-8ºC or below. Avoid multiple freeze-thaw cycles.
Anwendungsbeschreibung: WB: 1:500-1:3000.*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications., ICC/IF: 1:100-1:1000.*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other...
 TNF alpha antibody detects TNF alpha protein by western blot analysis.
A. 30 μg NCI-H929 whole cell lysate/extract
12% SDS-PAGE
TNF alpha antibody (GTX110520) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 TNF alpha antibody detects TNF alpha protein at cytoplasm by immunofluorescent analysis. Sample: Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500. Blue: Hoechst 33342 staining.
 TNF alpha antibody detects TNF alpha protein at membrane on human gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human gastric carcinoma. TNF alpha antibody (GTX110520) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
 TNF alpha antibody detects TNF alpha protein at membrane by immunofluorescent analysis. Sample: jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500. Blue: Hoechst 33342 staining.
 TNF alpha antibody detects TNF alpha protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 TNF alpha antibody detects TNF alpha protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TNF alpha antibody (GTX110520) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 TNF alpha antibody detects TNF alpha protein at cell membrane in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. TNF alpha antibody (GTX110520) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 TNF alpha antibody detects TNF alpha protein at cytoplasm on human esophagus carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus carcinoma . TNF alpha antibody (GTX110520) diluted at 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
 Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 TNF alpha antibody detects TNF alpha protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.