TNF alpha antibody, IgG, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX-GTX110520
Artikelname: TNF alpha antibody, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX-GTX110520
Hersteller Artikelnummer: GTX110520
Alternativnummer: GTX110520-25,GTX110520-100
Hersteller: Genetex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB, ICC/IF, IHC-P
Spezies Reaktivität: Human, Mouse, Rat, Bovine
Immunogen: Full length human TNF alpha Recombinant protein.
Konjugation: Unconjugated
Alternative Synonym: APC1 protein, cachectin, DIF, TNF, macrophage-derived, TNF, monocyte-derived, TNF-a, TNFA, TNF-alpha, TNFSF2, TNLG1F, tumor necrosis factor, tumor necrosis factor ligand 1F, tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Klonalität: Polyclonal
Konzentration: 0.04 mg/ml (Please refer to the vial label for the specific concentration.)
Isotyp: IgG
NCBI: 7124
UniProt: P01375
Puffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: tumor necrosis factor
Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
PMID: 24666525">  The WB analysis of TNF alpha antibody was published by Lee AS and colleagues in the journal Cardiovasc Diabetol in 2014.PMID: 24666525
 Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 TNF alpha antibody detects TNF alpha protein at membrane on human gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human gastric carcinoma.
TNF alpha antibody (GTX110520) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
 TNF alpha antibody detects TNF alpha protein at membrane by immunofluorescent analysis.
Sample: jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500.
Blue: Hoechst 33342 staining.
 TNF alpha antibody detects TNF alpha protein by western blot analysis.
A. 30 μg NCI-H929 whole cell lysate/extract
12% SDS-PAGE
TNF alpha antibody (GTX110520) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 TNF alpha antibody detects TNF alpha protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 TNF alpha antibody detects TNF alpha protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TNF alpha antibody (GTX110520) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 TNF alpha antibody detects TNF alpha protein at cell membrane in mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
TNF alpha antibody (GTX110520) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 TNF alpha antibody detects TNF alpha protein at cytoplasm on human esophagus carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus carcinoma .
TNF alpha antibody (GTX110520) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
 Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 TNF alpha antibody detects TNF alpha protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 TNF alpha antibody detects TNF alpha protein at cytoplasm by immunofluorescent analysis.
Sample: Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500.
Blue: Hoechst 33342 staining.