FIS1 antibody, IgG, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX-GTX111010
Artikelname: FIS1 antibody, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX-GTX111010
Hersteller Artikelnummer: GTX111010
Alternativnummer: GTX111010-25,GTX111010-100
Hersteller: Genetex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB, ICC/IF, IHC-P
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Full length human FIS1 Recombinant protein.
Konjugation: Unconjugated
Alternative Synonym: CGI-135, FIS1 homolog, fission 1 (mitochondrial outer membrane) homolog, H_NH0132A01.6, hFis1, mitochondrial fission 1 protein, mitochondrial fission molecule, tetratricopeptide repeat domain 11, tetratricopeptide repeat protein 11, TPR repeat protein 11, TTC11
Klonalität: Polyclonal
Konzentration: 1.2 mg/ml (Please refer to the vial label for the specific concentration.)
Isotyp: IgG
NCBI: 51024
UniProt: Q9Y3D6
Puffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: fission, mitochondrial 1
Anwendungsbeschreibung: WB: 1:500-1:20000. ICC/IF: 1:100-1:2000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
 Sample (30 μg of whole cell lysate)
A: Raji
15% SDS PAGE
GTX111010 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 FIS1 antibody detects FIS1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Sample (50 μg of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX111010 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: FIS1 protein stained by FIS1 antibody (GTX111010) diluted at 1:2000.
Red: MitoTrackerR Red CMXRos, a mitochondria tracker.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
 TTC11 antibody detects TTC11 protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
TTC11 antibody (GTX111010) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
 Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:10000.
 Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using FIS1(GTX111010) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
 Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.