FIS1 antibody

Artikelnummer: GTX-GTX111010
Artikelname: FIS1 antibody
Artikelnummer: GTX-GTX111010
Hersteller Artikelnummer: GTX111010
Hersteller: Genetex
Wirt: Rabbit
Applikation: WB, IF, ICC, IHC-P
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Full length human FIS1 Recombinant protein.
Konjugation: IgG
Alternative Synonym: Fission, Mitochondrial 1,Cgi-135,Ttc11,Fis1
Klonalität: Polyclonal
Konzentration: 1.2 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 17
Isotyp: IgG
NCBI: 51024
UniProt: Q9Y3D6
Puffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Formulierung: Liquid
Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4-8ºC. For long-term storage, aliquot and store at -20-8ºC or below. Avoid multiple freeze-thaw cycles.
Anwendungsbeschreibung: WB: 1:500-1:20000.*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications., ICC/IF: 1:100-1:2000.*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other...
 Sample (30 μg of whole cell lysate)
A: Raji
15% SDS PAGE
GTX111010 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 FIS1 antibody detects FIS1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 FIS1 antibody detects FIS1 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
15% SDS-PAGE
FIS1 antibody (GTX111010) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Sample (50 μg of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX111010 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: FIS1 protein stained by FIS1 antibody (GTX111010) diluted at 1:2000. Red: MitoTrackerR Red CMXRos, a mitochondria tracker. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
 TTC11 antibody detects TTC11 protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
TTC11 antibody (GTX111010) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
 Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:10000.
 Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using FIS1(GTX111010) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
 Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.