Human IL2 protein, His tag (active), Unconjugated

Produktübersicht

• Artikelname: Human IL2 protein, His tag (active), Unconjugated
• Artikelnummer: GTX00092-PRO
• Hersteller Artikelnummer: GTX00092-pro
• Alternativnummer: GTX00092-PRO-10
• Hersteller: GeneTex
• Kategorie: Proteine/Peptide
• Applikation: FA
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: interleukin 2 , IL-2 , TCGF , lymphokine

Produkteigenschaften

• NCBI: 3558
• UniProt: P60568
• Puffer: Reconstitute with 10mM PBS (pH7.4) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.6), 5% Trehalose, no preservatives.
• Expression System: HEK293 cells
• Formulierung: Lyophilized powder
• Sequenz: N-terminal His-Tag, Ala21~Thr153 (NP_000577.2)

Anwendungsbeschreibung

• Anwendungsbeschreibung: IL2 (Interleukin-2) is a cytokine produced by T-cells in response to antigenic or mitogenic stimulation. IL2 is a type of signaling molecule in the immune system, that is required for both T-cell and B-cell proliferation and other activities crucial to regulation of the immune response. Recombinant human IL2 shares 56% AA sequence identity with mouse IL2, suggesting the exist of cross-species activity. Therefore, in order to detect the bioactivity of rhIL2, a cell proliferation assay has been conducted using CTLL-2 mouse cytotoxic T cells. Briefly, CTLL-2 cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well with or without the addition of various concentrations of IL2. After incubated for 48h, cells were observed by inverted microscope. Proliferation of CTLL-2 cells after incubation with IL2 for 48h observed by inverted microscope.