Human IL2 protein, His tag (active), Unconjugated

Product Overview

• Article Name: Human IL2 protein, His tag (active), Unconjugated
• Biozol Catalog Number: GTX00092-PRO
• Supplier Catalog Number: GTX00092-pro
• Alternative Catalog Number: GTX00092-PRO-10
• Manufacturer: GeneTex
• Category: Proteine/Peptide
• Application: FA
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: interleukin 2 , IL-2 , TCGF , lymphokine

Product Properties

• NCBI: 3558
• UniProt: P60568
• Buffer: Reconstitute with 10mM PBS (pH7.4) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.6), 5% Trehalose, no preservatives.
• Expression System: HEK293 cells
• Form: Lyophilized powder
• Sequence: N-terminal His-Tag, Ala21~Thr153 (NP_000577.2)

Application Notes

• Application Notes: IL2 (Interleukin-2) is a cytokine produced by T-cells in response to antigenic or mitogenic stimulation. IL2 is a type of signaling molecule in the immune system, that is required for both T-cell and B-cell proliferation and other activities crucial to regulation of the immune response. Recombinant human IL2 shares 56% AA sequence identity with mouse IL2, suggesting the exist of cross-species activity. Therefore, in order to detect the bioactivity of rhIL2, a cell proliferation assay has been conducted using CTLL-2 mouse cytotoxic T cells. Briefly, CTLL-2 cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well with or without the addition of various concentrations of IL2. After incubated for 48h, cells were observed by inverted microscope. Proliferation of CTLL-2 cells after incubation with IL2 for 48h observed by inverted microscope.