Human FNDC5 protein, His tag (active), Unconjugated

Produktübersicht

• Artikelname: Human FNDC5 protein, His tag (active), Unconjugated
• Artikelnummer: GTX00150-PRO
• Hersteller Artikelnummer: GTX00150-pro
• Alternativnummer: GTX00150-PRO-10
• Hersteller: GeneTex
• Kategorie: Proteine/Peptide
• Applikation: FA
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: fibronectin type III domain containing 5 , FRCP2 , irisin

Produkteigenschaften

• NCBI: 252995
• UniProt: Q8NAU1
• Puffer: Reconstitute with 10mM PBS (pH7.4) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.6), 5% Trehalose, no preservatives.
• Expression System: HEK293 cells
• Formulierung: Lyophilized powder
• Sequenz: N-terminal His-Tag, Asp32~Glu143 (NP_001165411.2)

Anwendungsbeschreibung

• Anwendungsbeschreibung: Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. It was reported that FNDC5 significantly decreased cell number, migration and viability through apoptosis in malignant MDA-MB-231 cells. Thus MDA-MB-231 cells were seeded overnight at a density of 5000 cells/well, and treated with or without various concentrations of FNDC5 for 48h, then MDA-MB-231 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37C. Cell apoptosis of MDA-MB-231 cells after incubation with FNDC5 for 48h observed by inverted microscope.