Human FNDC5 protein, His tag (active), Unconjugated

Product Overview

• Article Name: Human FNDC5 protein, His tag (active), Unconjugated
• Biozol Catalog Number: GTX00150-PRO
• Supplier Catalog Number: GTX00150-pro
• Alternative Catalog Number: GTX00150-PRO-10
• Manufacturer: GeneTex
• Category: Proteine/Peptide
• Application: FA
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: fibronectin type III domain containing 5 , FRCP2 , irisin

Product Properties

• NCBI: 252995
• UniProt: Q8NAU1
• Buffer: Reconstitute with 10mM PBS (pH7.4) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.6), 5% Trehalose, no preservatives.
• Expression System: HEK293 cells
• Form: Lyophilized powder
• Sequence: N-terminal His-Tag, Asp32~Glu143 (NP_001165411.2)

Application Notes

• Application Notes: Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. It was reported that FNDC5 significantly decreased cell number, migration and viability through apoptosis in malignant MDA-MB-231 cells. Thus MDA-MB-231 cells were seeded overnight at a density of 5000 cells/well, and treated with or without various concentrations of FNDC5 for 48h, then MDA-MB-231 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37C. Cell apoptosis of MDA-MB-231 cells after incubation with FNDC5 for 48h observed by inverted microscope.